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Simple, cheap loading dye for Agarose gel electrophoresis (page 2 of 2) The ideal dye would be dark when you load it (so that's easy to see) and barely visible when the gel is finished running (so that's is easy to image without dye spots).
 Click image for larger
The Fisher 5x Sucrose Dye has a few essential characteristics:- As I mentioned above, the dye coloring is way too concentrated. But this actually turns out to be a benefit, because we can dilute the dye like crazy, and one cheap little bottle lasts for a looooong time.
- The Sucrose Dye actually contains three colors (red, blue, and purple) (see photo above), which travel at different rates. These three colors make the sample dark when we load it and lighter as the gel runs (because the three colors separate leaving the darkness of each single color at about a third its original darkness). So it's pretty easy to make this loading dye dark when you load it and light when you image it.
- Since density component of the dye is sucrose, you can pipette the dye more easily than the more viscous dyes that contain glycerol or ficoll.
My recipe you need: Dilute your Fisher 5x Sucrose Dye into 40% sucrose. This dilution into clear sucrose water will lower the concentration of the dye coloring, while maintaining the density needed to make your sample fall gently into the well.
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